A modified fungicide based media for the isolation of Phytophthora cinnomomi Rands. causing avocado root rot

Abstract

The commonly chosen medium for isolating Phytophthora is the P10VP medium, which contains pimaricin (10 mg), vancomycin (220 mg), and PCNB (pentachloronitrobenzene) (125 mg a.i.). Pimaricin has been a longstanding ingredient in the PIOVP medium for Phytophthora isolation. In this research, we designed a modified isolation medium with propiconazole as the primary component for isolating Phytophthora from infected plant samples. This modified medium was tested and validated for various Phytophthora species affecting different crops.