Modified QuEChERS technique and analysis methodology using UHPLC for residue analysis of thiacloprid and emamectin benzoate in chilli

Abstract

The presence of insecticide residues in chilli fruits limits the export and poses a health risk to consumers. Thus, it is necessary to develop quick and effective methods for conducting residue analysis of commonly used insecticides. The present research validates a modified QuEChERS technique for analysing thiacloprid and emamectin benzoate residues in chilli using UHPLC. The proposed methods recovered thiacloprid and emamectin benzoate by 80.33-88% and 79.33-96%, respectively. LOQ for both the insecticides was 0.01 mg/kg. Matrix effect values were within an acceptable range of -20 to 20%. The developed methodology can be used for periodical monitoring of the residue levels of the target insecticides in chilli.