Biosafety assessment of nanemulsion of hexanal on Mallada boninensis Okamoto (Chrysopdae: Neuroptera)

Abstract

Experiments were carried out under laboratory conditions to assess the effect of nano emulsion of hexanal on egg and grub stage of green lacewing, Mallada boninensis Okamoto (Neuroptera: Chrysopidae) through laboratory bioassay. The results showed that, nanoemulsion of hexanal was found to cause egg hatchability up to 90.04 percent in higher concentrations of 0.06 percent. Egg hatchability at field recommended concentration of 0.02% was found maximum of 94.18 percent respectively. In addition nanoemulsion of hexanal at field recommended concentration 0.02% tested against grubs of M. boninensis results revealed that 6.67 percent grub mortality in dry film method at 48h after exposure it was least toxic to grub of M. boninensis. The maximum percent pupation and adult emergence was recorded in nanoemulsion of hexanal @ 0.02% showed 95.33 and 95.0 percent, respectively. The results clearly indicated that all the concentration of nano formulation of hexanal was harmless to M. boninensis which recorded a
mortality of < 30 percent as per the threshold prescribed by IOBC.