Translocation of Bacillus thuringiensis kurstaki strain HD-1in brinjal seedling by different inoculation methods

Abstract

In the present study, Btk strain HD1 was inoculated at a concentration of 108 cfu/ml to brinjal in three methods under pot culture studies. The methods include seed treatment, soil application and foliar spray. Btk strain HD1 from leaves of seedlings was recovered in all the application methods. In seed treatment,Btk HD1 was detected in leaves after 30th day after inoculation while in soil application and foliar spray the Btk HD1 was detected upto 10th DAI. Further the colonies recovered from leaf tissues collected from three inoculation methods were examined for morphological, spore crystal formation andcry1 gene amplification. This is the first kind of report regarding the endophytic ability of Btk strain HD1 in brinjal by which the approach could be a possible way for the development of insect resistant crop for sustainable agriculture.