Molecular diagnosis of Chilli veinal mottle virus causing mosaic in Capsicum (Capsicum annum var. grossum Sendt)

Abstract

Viral diseases are considered as the major limiting factors in Capsicum (Capsicum annum var. grossum Sendt) production and Chili veinal mottle virus (ChiVMV) is one of the important viruses, which decreases yield significantly. Surveys were conducted during rabi 2015-16 to determine the prevalence of incidence of mosaic disease in major capsicum growing districts namely, Chikkaballapura, Kolar, Bengaluru rural and Ramanagar. The per cent incidence of mosaic disease based on symptoms in field was noticed highest in Ramanagar (54.85 %) and the least incidence of mosaic disease was observed in Chikkaballapura (26.85 %). The symptoms includes yellowing, mosaic and mottling and the virus was preliminarily confirmed by DAC-ELISA. Among the 40 samples, 30 samples were reacted positively with ChiVMV antiserum and later it was maintained in Datura metel and the viral genome was analysed. Further, the diagnosis of virus causing mosaic disease in capsicum by RT-PCR, revealed that the percentage of nucleotide homology of ChiVMV- Kolar (KOL-1) isolate found to be 93.81 per cent and the lowest nucleotide homology of ChiVMV-KOL-1 isolate found 78.31 per cent and percentage of nucleotide homology of Chikkaballapura (CHB-1) isolate was found to be 93.97 per cent and the lowest nucleotide homology of ChiVMV-CHB-1isolate found to be 81.96 per cent.